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Auteur Saidi Amal |
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Isolement et caractérisation de la mycoflore nuisible et bénéfique associée au blé / Saidi Amal (2024)
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Titre : Isolement et caractérisation de la mycoflore nuisible et bénéfique associée au blé Type de document : texte imprimé Auteurs : Saidi Amal, Auteur Année de publication : 2024 Importance : 151 p Présentation : couv.ill.en coul. Format : 30cm+CD ISBN/ISSN/EAN : 004A/THE.DOC/SAI Langues : Anglais (eng) Mots-clés : Wheat, Mycoflora, biodiversity, phytopathogenicity, Akanthomyces, biological control, resistance, triazol. Résumé : This work concerned the isolation and characterization of mycoflora associated with wheat. In the first part, a study of the biodiversity of fungi isolated from wheat grains and plants was carried out. A total of 26 samples of hard and soft wheat grains of different varieties and wheat plants are taken during the 2018-2019 agricultural season from the three wilayas Bouira, Setif and Bordj Bou Arreridj. After isolation and characterization of these fungi. The colonization rates of grains by fungi are 93.17% for durum wheat grains, 88.88% for soft wheat grains and 100% for wheat plants. This study enabled the identification of 33 fungal genera. This shows the biodiversity of the mycoflora associated with wheat, with a dominance of the Alternaria genus in wheat kernels and the Fusarium genus on seedlings.
In the second part of our study, we focused on the harmful mycoflora of wheat. The strains used were species of the genus Fusarium (F. graminearum, F. culmorum and F. lateritium), isolated on DCPA medium supplemented with fungicides. Strains of F. graminearum and F. culmorum showed some resistance to difenoconazole and triadimenol, and were also highly aggressive to wheat.
In the third part, we focused on beneficial mycoflora. A strain of Akanthomyces muscarius was isolated from wheat grains and identified by morphological and molecular tests. The strain was then tested for its ability to control Thaumetopoea pityocampa larvae using dose-mortality tests, and for its ability to control certain phytopathogenic fungi using direct confrontation tests. Dose-mortality tests at three concentrations of A. muscarius revealed a mortality of 88.23% after 10 days for the concentration of 2.3 x 106 conidia ml-1. Our isolate also showed antifungal activity against phytopathogenic fungi, with inhibition rates ranging from 39.61% to 52.94%. The A. muscarius strain has a dual biocontrol potential against plant pests and diseases.En ligne : https://www.pnst.cerist.dz/recherche.php?ti=&eb=101&mc=&dm=0&au=Saidi+Amal&ln=&d [...] Isolement et caractérisation de la mycoflore nuisible et bénéfique associée au blé [texte imprimé] / Saidi Amal, Auteur . - 2024 . - 151 p : couv.ill.en coul. ; 30cm+CD.
ISSN : 004A/THE.DOC/SAI
Langues : Anglais (eng)
Mots-clés : Wheat, Mycoflora, biodiversity, phytopathogenicity, Akanthomyces, biological control, resistance, triazol. Résumé : This work concerned the isolation and characterization of mycoflora associated with wheat. In the first part, a study of the biodiversity of fungi isolated from wheat grains and plants was carried out. A total of 26 samples of hard and soft wheat grains of different varieties and wheat plants are taken during the 2018-2019 agricultural season from the three wilayas Bouira, Setif and Bordj Bou Arreridj. After isolation and characterization of these fungi. The colonization rates of grains by fungi are 93.17% for durum wheat grains, 88.88% for soft wheat grains and 100% for wheat plants. This study enabled the identification of 33 fungal genera. This shows the biodiversity of the mycoflora associated with wheat, with a dominance of the Alternaria genus in wheat kernels and the Fusarium genus on seedlings.
In the second part of our study, we focused on the harmful mycoflora of wheat. The strains used were species of the genus Fusarium (F. graminearum, F. culmorum and F. lateritium), isolated on DCPA medium supplemented with fungicides. Strains of F. graminearum and F. culmorum showed some resistance to difenoconazole and triadimenol, and were also highly aggressive to wheat.
In the third part, we focused on beneficial mycoflora. A strain of Akanthomyces muscarius was isolated from wheat grains and identified by morphological and molecular tests. The strain was then tested for its ability to control Thaumetopoea pityocampa larvae using dose-mortality tests, and for its ability to control certain phytopathogenic fungi using direct confrontation tests. Dose-mortality tests at three concentrations of A. muscarius revealed a mortality of 88.23% after 10 days for the concentration of 2.3 x 106 conidia ml-1. Our isolate also showed antifungal activity against phytopathogenic fungi, with inhibition rates ranging from 39.61% to 52.94%. The A. muscarius strain has a dual biocontrol potential against plant pests and diseases.En ligne : https://www.pnst.cerist.dz/recherche.php?ti=&eb=101&mc=&dm=0&au=Saidi+Amal&ln=&d [...] Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 004A/THE.DOC 004A/THE.DOC/SAI Relié Thèse de Doctorat Thèse de Doctorat Disponible