Influence of gender, obesity, and muscle lipase activity on intramyocellular lipids in sedentary individuals / Cédric Moro  

Public ISBD Faire une suggestion d'achat Titre : Influence of gender, obesity, and muscle lipase activity on intramyocellular lipids in sedentary individuals Type de document : document électronique Auteurs : Cédric Moro, Auteur ; Jose E. Galgani, Auteur ; Lanchi Luu, Auteur ; Magdalena Pasarica, Auteur ; Aline Mairal, Auteur ; Sudip Bajpeyi, Auteur ; Gerd Schmitz, Auteur ; Dominique Langin, Auteur ; Gerhard Liebisch, Auteur ; Steven R. Smith, Auteur Année de publication : 2009 Langues : Anglais (eng) Catégories : 571 Physiologie générale et sujets connexes Physiology and related subjects Tags : Adult  Aged  Muscle,Skeletal  Obesity  Sex Factors  Triglycerides  Ceramides  Diglycerides  Female  Humans  Insulin Resistance  Lipase  Male  Middle Aged  Sciences du Vivant [q-bio]  571Biochimie,  Biologie Moléculaire Index. décimale : 571 Résumé : Obesity and type 2 diabetes are associated with elevated intramyocellular lipids (IMCLs) and insulin resistance. OBJECTIVE: We tested the hypothesis that skeletal muscle lipases activity could influence IMCL content (including diacylglycerol and ceramides). DESIGN AND PATIENTS: The present study included 48 subjects with a wide range of age (19-68 yr) and body mass index (20-45 kg/m(2)) who underwent skeletal muscle biopsy, dual-energy x-ray absorptiometry and a hyperinsulinemic euglycemic clamp. MAIN OUTCOME MEASURES: Insulin sensitivity by hyperinsulinemic clamp, and intramyocellular triacylglycerol (IMTG), diacylglycerol (DAG), and ceramides content, and triacylglycerol and diacylglycerol hydrolase activities were measured in biopsies of vastus lateralis. IMCL was measured by (1)H-magnetic resonance spectroscopy in a subgroup of 25 subjects. Multivariate regression analyses were performed to identify the main predictors of IMCL. RESULTS: Body fat was the main predictor of IMTG independently of the method and the type of muscle; IMTG concentration was higher in females vs. males and obese vs. nonobese subjects. Muscle DAG and ceramides concentrations were elevated in obese and type 2 diabetic subjects and were not related to body fat and fasting free fatty acids, whereas a direct association with the ratio of diacylglycerol hydrolase to triacylglycerol hydrolase activity (an index of incomplete triacylglycerol hydrolysis) was observed, which explained 54 and 38% of the variance in DAG and ceramides (P En ligne : https://hal.science/inserm-00410118v1 Format de la ressource électronique : HTML Influence of gender, obesity, and muscle lipase activity on intramyocellular lipids in sedentary individuals [document électronique] / Cédric Moro, Auteur ; Jose E. Galgani, Auteur ; Lanchi Luu, Auteur ; Magdalena Pasarica, Auteur ; Aline Mairal, Auteur ; Sudip Bajpeyi, Auteur ; Gerd Schmitz, Auteur ; Dominique Langin, Auteur ; Gerhard Liebisch, Auteur ; Steven R. Smith, Auteur . - 2009. Langues : Anglais (eng) Catégories : 571 Physiologie générale et sujets connexes Physiology and related subjects Tags : Adult  Aged  Muscle,Skeletal  Obesity  Sex Factors  Triglycerides  Ceramides  Diglycerides  Female  Humans  Insulin Resistance  Lipase  Male  Middle Aged  Sciences du Vivant [q-bio]  571Biochimie,  Biologie Moléculaire Index. décimale : 571 Résumé : Obesity and type 2 diabetes are associated with elevated intramyocellular lipids (IMCLs) and insulin resistance. OBJECTIVE: We tested the hypothesis that skeletal muscle lipases activity could influence IMCL content (including diacylglycerol and ceramides). DESIGN AND PATIENTS: The present study included 48 subjects with a wide range of age (19-68 yr) and body mass index (20-45 kg/m(2)) who underwent skeletal muscle biopsy, dual-energy x-ray absorptiometry and a hyperinsulinemic euglycemic clamp. MAIN OUTCOME MEASURES: Insulin sensitivity by hyperinsulinemic clamp, and intramyocellular triacylglycerol (IMTG), diacylglycerol (DAG), and ceramides content, and triacylglycerol and diacylglycerol hydrolase activities were measured in biopsies of vastus lateralis. IMCL was measured by (1)H-magnetic resonance spectroscopy in a subgroup of 25 subjects. Multivariate regression analyses were performed to identify the main predictors of IMCL. RESULTS: Body fat was the main predictor of IMTG independently of the method and the type of muscle; IMTG concentration was higher in females vs. males and obese vs. nonobese subjects. Muscle DAG and ceramides concentrations were elevated in obese and type 2 diabetic subjects and were not related to body fat and fasting free fatty acids, whereas a direct association with the ratio of diacylglycerol hydrolase to triacylglycerol hydrolase activity (an index of incomplete triacylglycerol hydrolysis) was observed, which explained 54 and 38% of the variance in DAG and ceramides (P En ligne : https://hal.science/inserm-00410118v1 Format de la ressource électronique : HTML

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